Blood samples 10ml were collected from each animals at end of experiment by the heart cardiac puncture. Research article alloxan induced diabetes mellitus in. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Mice administered a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg showed progression of typei diabetes symptoms figure 2. Changes in blood glucose f profile of alloxaninduced diabetic rabbits for 26. The first group 5 rabbits was used as control and fed with standard chow and tap water.
Diabetic atherosclerosis was induced in rabbit iliofemoral arteries by injecting alloxan 100 mgkg, injuring the arteries using a balloon, and feeding with a 0. Diabetes mellitus, rabbits, alloxan abstract diabetes mellitus dm a disease which is characterized by hyperglycemia, lipoprotein abnormalities and oxidative stress. These agents selectively destroy the langerhans islet. The present paper is an extension of this study of the mechanism of alloxan hypoglycemia.
In remaining ten rabbits, diabetes mellitus was induced by alloxan monohydrate 150 mgkg by intraperitoneal administration. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. This study undertaken to investigate the was beneficial effect of vitamin c and zinc on some biochemical parameter when induced diabetic in rabbits by alloxanrabbits groups. Pdf pathomorphological effects of alloxan induced acute. The results showed a significant improvement in the stress induced oxidation parameters by alloxaninduced diabetes in rabbits. As seen from the data in figure 2, fasting blood glucose levels were reduced versus vehicle treatment following two weeks of oral treatment with glyburide 39. The present study investigated metabolic changes and their relationship to vascular histopathological changes in the atherosclerotic arteries of rabbits with alloxaninduced diabetes. Xanthine oxidase inhibitor allopurinol prevents oxidative.
If the inline pdf is not rendering correctly, you can download the pdf file here. They also exhibit a variety of biological activities. Alloxan induced and insulinresistant diabetes mellitus affect semen parameters and impair spermatogenesis in male rats 108 african journal of reproductive health african journal of reproductive health vol. Antidiabetic effect of ipomoea batatas in normal and alloxan. The mechanisms of alloxan and streptozotocininduced diabetes. Alloxan and streptozotocin are toxic glucose analogues that preferentially. Attenuation of oxidative damage in alloxan induced. Mar 23, 2012 diabetes was induced by streptozotocin or alloxan injection. Attenuation of oxidative damage in alloxan induced diabetic. Alloxan induced diabetic rabbits n 28 were divided. In 1937 jacobs 1 found that alloxan 2 the ureide of mesoxalic acid produced fatal hypoglycemia when injected intravenously into rabbits. Experimental model of induction of diabetes mellitus in rats. Metformin was orally administered at 50 mgkg, while the extract was administered both to normal and diabetic rabbits at 100, 200 and 300 mgkg. The mechanism of alloxan and streptozotocin action in b cells.
May 24, 2014 mice administered a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg showed progression of typei diabetes symptoms figure 2. Bailey cc, bailey ot, leech rs 1946 diabetes mellitus in rabbits. Histopathological abnormalities of prolonged alloxaninduced. K p v o o evaluation and optimization of effectivedose. The mechanisms of alloxan and streptozotocininduced. The present study described the phytochemical profile of lavandula stoechas essential oils, collected in the area of aindraham northwest of tunisia, as well as their protective effects against alloxaninduced diabetes and oxidative stress in rat. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. The alloxan model of diabetes was first described in rabbits by dunn, sheehan and mcletchie in 1943. Alloxan monohydrate is a common drug used for developing experimental diabetes in animals, as streptozotocin, though less toxic than alloxan is considerably costlier. This study aimed to create diabetic animal models and to provide an exact and detailed account of alloxan induced diabetes in rabbits.
The anhydrous derivative ocnhco 2 co is also known as well as a dimeric derivative. Effect of trigonella foenum methi on fasting blood glucose levels in. This study was to create a longterm rabbit model of diabetes mellitus for medical studies of up to one year or longer and to evaluate the effects of chronic hyperglycemia on damage of major organs. Alloxaninduced diabetes, a common model for evaluating. The mechanism of alloxan and streptozotocin action in b. Alloxan, diabetes mellitus, long evans rat, induced diabetes, toxicity. Alloxan induced diabetic rabbits as animal model for diabetes. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. Hypoglycaemic effect was comparable to that of glibenclamide.
Essential oils samples were obtained from the aerial parts of the plant by hydrodistillation and analyzed by gcms. The present study was carried out to evaluate the antidiabetic activity of vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. The name is derived from allantoin, a product of uric acid excreted by the fetus into the allantois, and oxaluric acid derived from oxalic acid and urea, found in urine. It has been successfully induced in a variety of animal species.
Diabetes mellitus was induced by a 120 mgkg body weight intravenous injection of alloxan. Diabetes mellitus were induced in twenty four starved pregnant female rabbits by giving alloxan injected by one ml size syringe and in dose 150mg\kg for three days. Alloxan is one of the frequently used betacytotoxic agents for the induction of type1 diabetes mellitus in animal models and is the drug of choice in rabbits. Since oxidative stress is mediated by hyperglycemiainduced generation of free radical, it is supposed that compounds with hypoglycemic and antioxidative properties would be useful antidiabetic agents. Alloxan has been widely used to induce experimental dm in animals such as rabbits, mice and dogs with different grades of disease severity by varying the dose of alloxan used 8 causing alloxan. Similar experiments conducted by dunn, sheehan and mcletchie 3 confirmed the hypoglycemic action of alloxan. In the alloxan induced diabetic rats showed that extract at 500 mgkg decreased significantly fasting plasma glucose level by 61. In vivo penile erection length induced by drugs was measured in conscious rabbits. Comparative toxicity of alloxan, nalkylalloxans and ninhydrin to isolated pancreatic islets in vitro. Introduction diabetes mellitus diabetes is a group of metabolic diseases in which a person has high blood sugar, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is. Sar407899 dosedependently relaxed the precontracted corpora cavernosa in all species, with similar potency and efficacy in healthy vs diabetic rats, wky vs shr rats, healthy vs diabetic rabbits.
In animals, it can be induced by partial pancreatectomy or by the administration of diabetogenic drugs such as alloxan, streptozotocin, ditizona and antiinsulin serum. Since then, alloxan diabetes has been commonly utilized as an animal model of insulindependent diabetes mellitus iddm. Antidiabetic activity of aqueous extract of leptospermum. Effects of aqueous extract of alfalfa on hyperglycemia and. The evaluation of soybean extracts in alloxan induced diabetic rabbits. Study the effect of phytoesterol of ceratoina female rabbits. A single dose of alloxan monohydrate 100 mgkg was given intravenously to 20 young new zealand white rabbits.
When they finish that diet, ordinary food was given to those. Alloxaninduced and insulinresistant diabetes mellitus. On microscopic study these observers found necrosis of. Antidiabetic effect of ipomoea batatas in normal and. The severe diabetes produced by alloxan results in blood sugar levels equivalent to a total pancreatectomy, hence sulphonylureas such. Research article alloxan induced diabetes mellitus in wistar. Hypoglycemic and antioxidant effect of oleuropein in alloxan. They were randomly and equally divided into the control group c, n30, alloxaninduced group with dm dm, n30 and allopurinoltreated group with dm allo, n30. A total of 90 male and female japanese rabbits weighing 1. Alloxan induced diabetic rabbits as animal model for. Schuh wholeplant in normal and alloxaninduced diabetic rabbits.
Another 12 agematched normal rabbits were used as controls. Extracts were administered daily for 7d at doses of 200 and 400mgkg, p. Dec 18, 2007 alloxan has two distinct pathological effects. Pdf in present study hypoglycaemic effects of the crude powdered c. Alloxan exerts its diabetogenic action when it is administered parenterally. Diabetes is a disease that constitutes multiple sources of free radicals, thus oxidative stress is expected to have a double impact. Alloxan monohydrate is used to induce diabetes mellitus in albino rats in the dose of 120mgkg i.
Nov 29, 2015 models for insulin dependent diabetes mellitus iddm alloxan induced diabetes principlemechanism interacts with sulfhydryl groups and inhibit it following its uptake by the beta cells, alloxan interacts with sulfhydryl containing cellular components, particularly enzymes known to be essential for beta cell function. The present study was conducted to investigate antidiabetic efficacy of extracts of nigella sativa linn. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Streptozotocin stz is used for induction of type1 diabetes mellitus in animal models. Alloxaninduced diabetes mellitus how is alloxaninduced. Similarly, alazzawie and alhamdani 19 reported that dietary oleuropein in alloxan diabetic rabbits reduced plasma mda levels to the level of nondiabetic animals. The effect of the injection of diabetogenic doses of alloxan on blood sugar levels. Antidiabetic activity of vinca rosea extracts in alloxan. Altered glucose metabolism and hypoxic response in alloxan. The methanolic whole plant extract at high dose 500 mgkg exhibited significant antihyperglycemic activity than whole plant extract at low dose 300 mgkg in diabetic rats. It is better to standardize it first by jain et al. Blood glucose lowering activity of aloe based composition. The present study investigated metabolic changes and their relationship to vascular histopathological changes in the atherosclerotic arteries of rabbits with alloxan induced diabetes. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals.
The ethanol extract of fenugreek seeds significantly decreases the plasma malondialdehyde mda from 2. Hypoglycemic and antioxidant effect of oleuropein in alloxandiabetic rabbits article in life sciences 7812. Effect of trigonelline and ethanol extract of iraqi fenugreek. Modulation of hematological indices of normal and alloxan. Antidiabetic activity of bauhinia forficata extracts in. Alloxaninduced rats,antidiabetes, diabetes, ipomoea batatas, phytochemical analysis. Alloxan dose of 120, 140, 160 and 180 mg kg 1 body weight was given intraperitonealy to experimental rats in groups of 6 animals for each dose.
The best known druginduced diabetes model is the alloxan diabetes. The optimum effective dose of alloxan was found to be 140 mgkg bw for induction of stable diabetes in long evan rats. These are some of the earliest known organic compounds. The pdf file you selected should load here if your web browser has a pdf. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. Effect of trigonelline and ethanol extract of iraqi. Sir, we read with interest the article titled anomalies in alloxaninduced diabetic model. Alloxan induced rats,anti diabetes, diabetes, ipomoea batatas, phytochemical analysis. The responses are accompanied by corresponding inverse changes in. Histopathological abnormalities of prolonged alloxaninduced diabetes mellitus in rabbits, international. The effect, however, was more pronounced in diabetic animals in which administration for 4 weeks after alloxan induced diabetes, significantly reduced blood glucose levels. Erectile properties of the rhokinase inhibitor sar407899 in. Streptozotocin induced acute clinical effects in rabbits.
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